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    17 hours ago David Crotty

    We’re getting toward the end of the second volume of our Emerging Model Organisms series in Cold Spring Harbor Protocols, and November’s issue brings us a look at the Hawaiian Bobtail Squid and the genus Dioscorea, or True Yams. Euprymna scolopes, the Hawaiian Bobtail Squid (our cover model this month, see below) is a cephalopod that’s well-suited for study in the laboratory. E. scolopes is primarily studied in three contexts: 1) as a model for cephalopod development–the embryos and protective chorions are clear, making it amenable for the observations and manipulations common in other studied model systems 2) as a model of animal-bacteria symbioses with the luminous marine bacterium Vibrio fischeri 3) as a system for studying the interaction of tissues with light, as the squid features a specialized light organ.

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    ⇪ Preparation of Genomic DNA from Hawaiian Bobtail Squid (Euprymna scolopes) Tissue by Cesium Chloride Gradient Centrifugation (1 link)
    PN Lee, MJ McFall-Ngai, P Callaerts, HG de Couet
    ⇪ Confocal Immunocytochemistry of Embryonic and Juvenile Hawaiian Bobtail Squid (Euprymna scolopes) Tissues (1 link)
    PN Lee, MJ McFall-Ngai, P Callaerts, HG de Couet
    ⇪ Whole-Mount In Situ Hybridization of Hawaiian Bobtail Squid (Euprymna scolopes) Embryos with DIG-Labeled Riboprobes: I. DNA Template Preparation and In Vitro Transcription of Riboprobes (1 link)
    PN Lee, MJ McFall-Ngai, P Callaerts, HG de Couet
    ⇪ Whole-Mount In Situ Hybridization of Hawaiian Bobtail Squid (Euprymna scolopes) Embryos with DIG-Labeled Riboprobes: II. Embryo Preparation, Hybridization, Washes, and Immunohistochemistry (1 link)
    PN Lee, MJ McFall-Ngai, P Callaerts, HG de Couet
    ⇪ Culture of Hawaiian Bobtail Squid (Euprymna scolopes) Embryos and Observation of Normal Development (1 link)
    PN Lee, P Callaerts, HG de Couet
    ⇪ True Yams (Dioscorea): A Biological and Evolutionary Link between Eudicots and Grasses (1 link)
    HD Mignouna, MM Abang, R Asiedu, R Geeta
    ⇪ Yam (Dioscorea) Husbandry: Cultivating Yams in the Field or Greenhouse (1 link)
    HD Mignouna, MM Abang, R Asiedu, R Geeta
    ⇪ Culturing Meristematic Tissue and Node Cuttings from Yams (Dioscorea) (1 link)
    HD Mignouna, MM Abang, R Asiedu, R Geeta
    ⇪ Post-Flask Management of Yam (Dioscorea) Plantlets (1 link)
    HD Mignouna, MM Abang, R Asiedu, R Geeta
    ⇪ Producing Yam (Dioscorea) Seeds through Controlled Crosses (1 link)
    HD Mignouna, MM Abang, R Asiedu, R Geeta
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